Intermediate

Part:BBa_K931005

Designed by: Chris Clifford   Group: iGEM12_Nevada   (2012-09-29)

SBP - TBP

The starch-binding protein gene (CBM21) and the thiamine-binding protein gene (ThiT) were placed in sequence with each other so that ThiT is on the C-Terminus and CBM21 is on the N-Terminus. CBM21 comes from R. oryzae. It is part of an operon that codes for a two-domain enzyme, one of which binds starch molecules. This can be used to bind starch residues that are found in rice. ThiT comes from E.coli and plays a crucial role in the thiamine transport system by binding the thiamine (vitamin B1) molecule. The resulting protein complex from these two genes has a starch-binding domain and a thiamine binding domain that bind their specific substrates.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1
    Illegal XbaI site found at 16
    Illegal SpeI site found at 343
    Illegal PstI site found at 357
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1
    Illegal SpeI site found at 343
    Illegal PstI site found at 357
    Illegal NotI site found at 7
    Illegal NotI site found at 350
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 1
    Illegal suffix found in sequence at 343
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 1
    Illegal XbaI site found at 16
    Illegal SpeI site found at 343
    Illegal PstI site found at 357
    Illegal NgoMIV site found at 1235
    Illegal NgoMIV site found at 1262
    Illegal AgeI site found at 425
    Illegal AgeI site found at 645
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None